Cell Normalization Technology for High Content Screening          : The glass coverslip format with an array of over 10.000 micropatterns and a printed grid for easy localization.    Sensitivity & powerful quantification to cell-based assays and screening    Cell based  assay를 위한 New Micropattern Chip    144 micropatterns in 2cm x 2cm,170um thick gridded coverslip of glass     5 shapes (Disk, Crossbow, H, Y, L)  and 3 sizes (large, medium,small)     Straightforward analysis, reliable results and faster discovery    - Reduce cell to cell variability    - Control the position of cell compartments and protein networks    - Achieve simple and rapid image analysis    - Map a "standard" averaged cell and use it as a reference    The CYTOO technology offer a whole range of new possibilities for the scientist.       and some applications have already been well established, others are developing    Easy analysis of cell contractility in response to drugs    CYTOO Microfludic's Theory :              In standard culture conditions (left), the shape and extent of the  Golgi      apparatus (in red) vary extensively with cell shape. On micropatterns (right),      the shape and positioning of the Golgi apparatus is identical in all cells      (Dimitrov, Thery, Bornens). 셀이 마이크로패턴상에 심어지면 셀과 기질간의  점착성질의 접촉에 의한 기하학적인 재분배가 일어나 셀기관내에서의 재현성있는 분극화를 얻을 수 있으며, 셀의 위치, 모양, 극성 등을 확인하여 셀 내부 조직들의 variability를 감소시켜 cell을 normalization 시킴.   세계적으로 수많은 private laboratories, leading research centers 그리고 제약회사와 생명공학기업 에서 응용되어지고 있는 CYTOO Chip은 하나의 chip 에 20,000개가 넘는 점착성의 마이크로패턴이 새겨진 glass chip을 이용하여 단지 12 to 20 cells 만으로도 재현성과 민감도가 뛰어난 cell based assay & Screening이  가능.    Applications :   Cell Shape and Actin Cytoskeleton     Discover how micropatterns can help you study the cytoskeleton organization                                       Microtubule Network     Visualizing microtubule dynamics in a predetermined actin cytoskeleton architecture  Cell Polarity and Organelle Positioning     Control, quantify and predict organelle positioning  Cell Division and Mitotic Spindle Orientation     Control mitotic spindle orientation and analyze symmetric, asymmetric and multipolar cell divisions   Quantitative Cell Phenotyping of Cell Behavior     Example of WT and TTL null Mouse Embryonic Fibroblasts   The Endomembrane Network :  Quantifying the 3D organization of the various endomembrane     compartments under various drugtreatments Cardiomyocyte Mechanobiology    Sarcomeric organization in response to the cell adhesion microenvironmen      Description of Micropatterns and uses Art gallery : favourite images taken on our adhesive micropatterns  Selected Publications Build your Reference Cell   Build a universal and timeless Reference for all your cell assays   Choose the right micropatterns   The Starter Chip  : 실험에 맞는 마이크로패턴 결정하기 Fibronectin Patterns Cells   Applications Cell shape Polarization Division Contractility Division Custom  - 실험하고자하는 application에     맞추어 셀 type을 선별, 선정     하기위해 도안제작된 tool  - 12 standard micropatterns      (4 shapes in 3 sizes).     The optimal micropattern size for your cell type    Product Specifications   Substrate High quality low fluorescence glass   Chip size 20 x 20 mm²   Glass thickness 170 μm   Standard pattern types* Disk, Crossbow, H, Y   Standard pattern sizes*        Small           Medium        Large   Cell area       700 μm²        1100 μm²     1600 μm²   No. of micropatterns per chip       20,736          20,736         11,664   Micropatterns per block       12 x 12         12 x 12         9 x 9   Pitch between micropatterns        100 μm         100 μm        130 μm   Micropattern line width          6 μm             6 μm          8 μm   Localization grid (GPS) Yes   Adhesion protein Fibronectin**   Packaging Set of 6; vacuum sealed blister pack Ordering Information   CYTOO chips are packaged in an embossed clear   plastic 6 blister tray sealed with paper.   In this format CYTOO chips may be loaded directly   into a 6 well plate with no direct manipulation.      The CYTOO chamber uses a      patented magnetic system (from      Live Cell Instruments) for easy      assembly.   The CYTOOchamber has the same footprint as standard   35mm culture dishes and therefore will fit into all 35mm   microscope stage adaptors.   CYTOOchambers are available in 1well and 4well formats.   We are currently updating our store to include the 4well      CYTOO PLATETM Microfludic multiwell Plate :  The standard glass bottom microplate format presenting thousands of micropatterns per well. CYTOOplates are ideal for pharma, biotech and academic screening center customers employing  high content cell- based assays for research, drug safety, and drug screening applications     - Glass-bottom microplates   - 96, 384-well format   - Structures for fluorescence applications with reduced background and minimum scattered light.   - Over 3,000 micropatterns. / well  A new dimension to your HCA/HCS assays: ?    - Powerful assay standardization    - Reproducible & sensitive cell phenotype quantification    - Straightfoward image Analysis   Cells on micropatterns   The Starter Plate  : 실험에 맞는 마이크로패턴 결정하기   실험하고자하는 application에   맞추어 셀 type을 선별, 선정   하기위해 도안제작된 tool   5 5 standard patterns in 2 sizes.   Columns 1 and 12 are homo-   geneously covered with adhesive   protein where your cells will   behave as if they were on a   conventional cell culture surface.