img1.png


  HOME>  PRODUCTS>  CYTOO

 

Cell Normalization Technology for High Content Screening
         : The glass coverslip format with an array of over 10.000 micropatterns and a printed grid for easy localization.

   Sensitivity & powerful quantification to cell-based assays and screening

   Cell based  assay New Micropattern Chip

   144 micropatterns in 2cm x 2cm,170um thick gridded coverslip of glass

    5 shapes (Disk, Crossbow, H, Y, L)  and 3 sizes (large, medium,small)

    Straightforward analysis, reliable results and faster discovery
   -
Reduce cell to cell variability
   - Control the position of cell compartments and protein networks
   - Achieve simple and rapid image analysis
   - Map a "standard" averaged cell and use it as a reference

   The CYTOO technology offer a whole range of new possibilities for the scientist.

      and some applications have already been well established, others are developing

   Easy analysis of cell contractility in response to drugs

 img002.gif  CYTOO Microfludic's Theory :

       

     In standard culture conditions (left), the shape and extent of the  Golgi
     apparatus (in red) vary extensively with cell shape. On micropatterns (right),
     the shape and positioning of the Golgi apparatus is identical in all cells
     (Dimitrov, Thery, Bornens).

ũϻ ɾ   ˿
й谡 Ͼ ִ бȭ
, ġ, , ؼ ȮϿ variability ҽ cell normalization Ŵ.
 

private laboratories, leading research centers
׸ ȸ б Ǿ ִ CYTOO
Chip ϳ chip 20,000 Ѵ ũ
glass chip ̿Ͽ 12 to 20 cells ε

ΰ پ cell based assay & Screening
  .

 

 

 img002.gif  Applications : 

 Cell Shape and Actin Cytoskeleton

    Discover how micropatterns can help you study the cytoskeleton organization                                     

 Microtubule Network

    Visualizing microtubule dynamics in a predetermined actin cytoskeleton architecture

 Cell Polarity and Organelle Positioning

    Control, quantify and predict organelle positioning

Application

 Cell Division and Mitotic Spindle Orientation

    Control mitotic spindle orientation and analyze symmetric, asymmetric and multipolar cell divisions 

 Quantitative Cell Phenotyping of Cell Behavior

    Example of WT and TTL null Mouse Embryonic Fibroblasts 

 The Endomembrane Network :  Quantifying the 3D organization of the various endomembrane
    compartments under various drugtreatments

Cardiomyocyte Mechanobiology

   Sarcomeric organization in response to the cell adhesion microenvironmen   

 

Description of Micropatterns and uses

micropattern

Art gallery : favourite images taken on our adhesive micropatterns

Art Gallery with CYTOO

 Selected Publications

Papers

Build your Reference Cell

  Build a universal and timeless Reference for all your cell assays

 

 

 

 

  

 

 

 

  Choose the right micropatterns
  The Starter Chip  : 迡 ´ ũ ϱ

Fibronectin Patterns

Cells

 

Applications

Cell shape

Polarization

Division

Contractility

Division

Custom

 - ϰϴ application
    ߾ type ,
    ϱ ۵ tool

 - 12 standard micropatterns
     (4 shapes in 3 sizes).

 

 

 

  The optimal micropattern size for your cell type
   Product Specifications

  Substrate

High quality low fluorescence glass

  Chip size

20 x 20 mm²

  Glass thickness

170 m

  Standard pattern types*

Disk, Crossbow, H, Y

  Standard pattern sizes*

       Small           Medium        Large

  Cell area

      700         1100      1600

  No. of micropatterns per chip

      20,736          20,736         11,664

  Micropatterns per block

      12 x 12         12 x 12         9 x 9

  Pitch between micropatterns

       100 m         100 m        130 m

  Micropattern line width

         6 m             6 m          8 m

  Localization grid (GPS)

Yes

  Adhesion protein

Fibronectin**

  Packaging

Set of 6; vacuum sealed blister pack

 

 

Ordering Information

  CYTOO chips are packaged in an embossed clear
  plastic 6 blister tray sealed with paper.
  In this format CYTOO chips may be loaded directly
  into a 6 well plate with no direct manipulation.

 

   The CYTOO chamber uses a
     patented magnetic system (from
     Live Cell Instruments) for easy
     assembly.



  
The CYTOOchamber has the same footprint as standard   35mm culture dishes and therefore will fit into all 35mm   microscope stage adaptors.

  CYTOOchambers are available in 1well and 4well formats.   We are currently updating our store to include the 4well

 

 

 

 img002.gif  CYTOO PLATETM Microfludic multiwell Plate :

 The standard glass bottom microplate format presenting thousands of micropatterns per well.

CYTOOplates are ideal for pharma, biotech and academic screening center customers employing
 high content cell- based assays for research, drug safety, and drug screening applications
 

  - Glass-bottom microplates

  - 96, 384-well format

  - Structures for fluorescence applications with reduced background and minimum scattered light.

  - Over 3,000 micropatterns. / well

 A new dimension to your HCA/HCS assays: ?
   - Powerful assay standardization
   - Reproducible & sensitive cell phenotype quantification
   - Straightfoward image Analysis

 

 

  Cells on micropatterns
  The Starter Plate  : 迡 ´ ũ ϱ

  ϰϴ application
  ߾ type ,
  ϱ ۵ tool

  5 5 standard patterns in 2 sizes.

  Columns 1 and 12 are homo-
  geneously covered with adhesive
  protein where your cells will
  behave as if they were on a   conventional cell culture surface.

 

 

 

 

bar002.png