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CYTOO

  Cell Normalization Technology for High Content Screening

      The glass coverslip format with an array of over 10.000 micropatterns and a printed grid for easy localization.

      Sensitivity & powerful quantification to cell-based assays and screening

      5 shapes (Disk, Crossbow, H, Y, L)  and 3 sizes (large, medium,small)

     144 micropatterns in 2cm x 2cm,170um thick gridded coverslip of glass

     Easy analysis of cell contractility in response to drugs

 

 CYTOO Microfludic's Theory :  

  In standard culture conditions (left), the shape and extent of the  Golgi apparatus (in red) vary extensively with cell shape.

  On micropatterns (right), the shape and positioning of the Golgi apparatus is identical in all cells  (Dimitrov, Thery, Bornens).

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ִ бȭ , ġ, , ؼ ȮϿ

variability ҽ cell normalization Ŵ.  

private laboratories, leading research centers ׸ ȸ б

Ǿ ִ CYTOO Chip ϳ chip 20,000 Ѵ ũ glass chip

̿Ͽ 12 to 20 cells ε ΰ پ cell based assay & Screening  .

                             

 Applications :      

 Cell Shape and Actin Cytoskeleton

    Discover how micropatterns can help you study the cytoskeleton organization                                     

 Microtubule Network

    Visualizing microtubule dynamics in a predetermined actin cytoskeleton architecture

 Cell Polarity and Organelle Positioning

    Control, quantify and predict organelle positioning

 Cell Division and Mitotic Spindle Orientation

    Control mitotic spindle orientation and analyze symmetric, asymmetric and multipolar cell divisions 

 Quantitative Cell Phenotyping of Cell Behavior

    Example of WT and TTL null Mouse Embryonic Fibroblasts 

 The Endomembrane Network :  Quantifying the 3D organization of the various endomembrane
    compartments under various drugtreatments

 Cardiomyocyte Mechanobiology

    Sarcomeric organization in response to the cell adhesion microenvironmen   

 Description of Micropatterns and uses

 Art gallery : favourite images taken on our adhesive micropatterns

 Selected Publications

 Build your Reference Cell

    Build a universal and timeless Reference for all your cell assays

 

 

                             

  Architectures       

            

                             

    Choose the right micropatterns 
 

Fibronectin Patterns

Cells

 

Applications

Cell shape

Polarization

Division

Contractility

Division

Custom

                             

    The Starter Chip  :

  迡 ´ ũ ϱ

 - ϰϴ application ߾ type , ϱ ۵ tool

 - 12 standard micropatterns (4 shapes in 3 sizes).

                             

The optimal micropattern size for your cell type

   Product Specifications

  Substrate

High quality low fluorescence glass

  Chip size

19.5 x 19.5 mm²

  Glass thickness

170 m

  Standard pattern types*

Disk, Crossbow, H, Y, L

  Standard pattern sizes*

       Small 

Medium 

Large

  Cell area

      700 m² 

1100 m²  

1600 m²

  No. of micropatterns per chip

      20,736  

20,736

11,664

  Micropatterns per block

      12 x 12

12 x 12    

  9 x 9

  Pitch between micropatterns

       100 m 

100 m

130 m

  Micropattern line width

         6 m  

   6 m

8 m

  Localization grid (GPS)

Yes

  Adhesion protein

Actibated

  Packaging

Set of 18; vacuum sealed blister pack

                             

Ordering Informatio

 

  CYTOO chips are packaged in an embossed clear plastic 6 blister tray

  sealed with paper.
  In this format CYTOO chips may be loaded directly into a 6 well plate

  with no direct manipulation.

 

 

    The CYTOO chamber uses a patented magnetic

    system (from  Live Cell Instruments) for easy

    assembly.

 The CYTOOchamber has the same footprint as standard  35mm culture

  dishes and therefore will fit into all 35mm microscope stage adaptors.

 CYTOOchambers are available in 1well and 4well formats.   

 We are currently updating our store to include the 4well

                             

   CYTOO PLATETM :

   Microfludic multiwell Plate

 The standard glass bottom microplate format presenting thousands of micropatterns per well.

 CYTOOplates are ideal for pharma, biotech and academic screening center customers employing high content

 cell- based assays for research, drug safety, and drug screening applications  

  - Glass-bottom microplates

  - 96, 384-well format

  - Structures for fluorescence applications with reduced background and minimum scattered light.

  - Over 3,000 micropatterns. / well

 A new dimension to your HCA/HCS assays:
  - Powerful assay standardization
  - Reproducible & sensitive cell phenotype quantification
  - Straightfoward image Analysis

 

 

Cells on micropatterns

  The Starter Plate  : 迡 ´ ũ ϱ

 

  ϰϴ application
  ߾ type ,  ϱ

   ۵ tool

  5 5 standard patterns in 2 sizes.

  Columns 1 and 12 are homo-
  geneously covered with adhesive
  protein where your cells will
  behave as if they were on a   conventional cell culture surface.

 

 

                             

 

 

 

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