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  Allied Monitor

  USDA/APHIS CERTIFIED JOHNE'S DISEASE CENTER

  Allied Monitor Inc is a privately owned diagnostic  laboratory and is the world’s  leading manufacturer

        of Paratuberculosis-Proptoplasmic, Ferric Mycobactin J, Antigen, and Absorben.

  Our reagents are distributed to media producers, kit manufacturers, research facilities and

         diagnostic laboratories in over 70 countries around the world.

 

         MODELS

   

   FERRIC MYCOBACTIN J

   Ferric Mycobactin J is an iron-chelated substance used as in culture media as a growth promoter for the isolation of

   mycobactin-dependent Mycobacterium.  

   Incorporation of MYCOBACTIN J into culture medium reduces the period of cultivation of approximately six to eight weeks

   for Mycobacterium paratuberculosis, and enhances the growth of strains of fastidious mycobacteria which otherwise could

   not be isolated.   

   Available in 2mg, 50mg, 100mg, 200mg and 1000mg sizes.  

 PARATUBERCULOSIS-PROTOPLASMIC

 ANTIGEN (PPA)
  
Used as the binding antigen in ELISA and

  AGID screening to detect antibodies

  produced against
  Mycobacterium avium
ssp.

  paratuberculosis (MAP).
  Available in 20mg, 40mg, and 80mg sizes.

  ABSORBEN (M.phlei)

  Used as the absorbing antigen for sera prior

  to screening with ELISA.

  Lyophilized product available in 100mg.

  Bulk Liquid form available in 1L.

  Larger volumes available upon request.

 

 

  POSITIVE REAGENTS   

  OVINE, BOVINE, and CAPRINE lyophilized

  sera used as a positive control in ELISA and

  AGID screening tests.

  Our positive controls are derived from

  naturally MAP infected adult ruminant

  species, and confirmed paratuberculosis

  positive by ELISA, AGID, culture, and

  necropsy. Available in 1mL.

  NEGATIVE REAGENTS

  OVINE, BOVINE, and CAPRINE lyophilized

  sera used as a negative control in ELISA and

  AGID screening tests. Controls are screened

  negative by ELISA and AGID tests.

  Available in 1mL.

 

 

 

 

  REFERENCES  

   Mycobacterium paratuberculosis sheep type strain in Uruguay: Evidence for a wider geographic distribution in South America

   Diagnostic Potential of Three Antigens from Geographically Different Regions of the World for the Diagnosis of Ovine Johne’s Disease in India

   Estimation of Mycobacterium avium subsp. paratuberculosis Growth Parameters: Strain Characterization and Comparison of Methods     

   Variation in Resistance of Mycobacterium paratuberculosis to Acid Environments as a Function of Culture Medium

   Cultivation of Mycobacterium paratuberculosis from Bovine Fecal Samples by Using Elements of the Roche MB Check System

   Iron Acquisition in Mycobacterium avium subsp. paratuberculosis

   Use of short-term culture for identi®cation of Mycobacterium avium subsp. paratuberculosis in tissue from Crohn's disease patients

   Evaluation of Mycobacterium avium subsp. paratuberculosis faecal culture protocols and media

   DIAGNOSIS OF PARATUBERCULOSIS IN CATTLE: MICROBIOLOGICAL CULTURE, SEROLOGY AND PCR

   First isolation of  MYCOBACTERIUM AVIUM SUBSP.  PARATUBERCULOSIS from wild GUANACOS (LAMA GUANICOE) on TIERRA DEL FUEGO Island

   Presence and characterization of Mycobacterium avium subspecies paratuberculosis from clinical and suspected cases of Crohn’s  disease

   and in the healthy human population in India

   Thiopurine Drugs Azathioprine and 6-Mercaptopurine Inhibit Mycobacterium paratuberculosis Growth In Vitro

   A Major Cell Wall Lipopeptide of Mycobacterium avium subspecies paratuberculosis

   Mycobactin-mediated intracellular iron acquisition from macrophages

   A murineoral model for Mycobacterium avium subs p.paratuberculosis infection and immuno modul at ion with Lactobacillus casei ATCC334   

   Fe3+/Fe2+ mycobactin-complex electrochemistry as an approach to determine mycobactin levels in urine.

   The Mycobacterium avium ssp.paratuberculosis specific mptD gene is required for maintenance of the metabolic homeostasis necessary

   for full virulence in mouse infections

   Mycobacterium paratuberculosis detection in cow’s milk in Argentina by immunomagnetic separation-PCR

   Mycobactin-mediated intracellular iron acquisition from macrophages

   Molecular Characterisation of Indian Strains of Mycobacterium avium subspecies paratuberculosis by Pulsed-Field Gel Electrophoresis

   Production and proteomic characterisation of purified protein derivative from Mycobacterium avium subsp. paratuberculosis

   Inactivation of Mycobacterium avium subsp. paratuberculosis during Cooking of Hamburger Patties

   Comparison of a quantitative real-time polymerase chain reaction (qPCR) with conventional PCR, bacterial culture and ELISA for

   detection of Mycobacterium avium subsp. paratuberculosis infection in sheep showing pathology of Johne’s disease

   THE MYCOBACTERIOLOGY OF INFLAMMATORY BOWEL DISEASE

   Novel Amphiphilic Cyclobutene and Cyclobutane cis-C18 Fatty Acid Derivatives Inhibit Mycobacterium avium subsp. paratuberculosis Growth

 

 

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