img1.png


  HOME>  PRODUCTS>  BIOLOGICS>  ULTRASONIC HOMOGENIZER

 

    BIOLOGICS 

 Ultrasonic Homogenizer Series  (Sonicators)    

 For over five decades, BioLogics laboratory equipment has been setting new standards for
     design innovation, performance, and reliability.

 The BioLogics family of Ultrasonic Homogenizers offer precision engineering with all the
     features necessary to create a total system for ultrasonic disruption.

 The Ultrasonic Homogenizers can disintegrate most cells, bacteria, spores or tissue.

 They can prepare an emulsion down to 1/100 of a micron homogenize "immiscible" liquids,
     accelerate enzymatic and chemical reactions, stimulate bacterial activity, disperse solids in
     liquids and degas liquids

  

            Features

  How it Works : 

 The ultrasonic power supply transforms line voltage to high frequency 20 kHz electrical energy.
 This electrical energy is transmitted to the probe where it is converted to mechanical energy.
 The vibrations from the probe are coupled to and intensified by the titanium tip.
 The probe vibrates in a longitudinal direction and transmits this motion to the titanium tip immersed in the solution.
 Cavitation results, in which microscopic vapor bubbles are formed momentarily and implode, causing powerful
   infinitesimal shock waves to radiate throughout the solution in proximity to the radiating face of the tip. 

  

 Contact vs. Non-Contact Homogenization Methods

 The CONTACT homogenization is the most common  method of processing a sample.
 The probe is immersed into the sample where the
 radiating face of the probe causes the sample
 to cavitate, quickly processing the sample.
 Small diameter probes such as the Stepped and
 Tapered Micro tips generate high intensity
 cavitation and are utilized when processing
 small volume samples.  Large diameter probes
 such as the Solid and Tapped tips generate low
 intensity cavitation and are utilized when
 processing large volume samples.

 The NON-CONTACT homogenization method is
 utilized when processing small volume samples
 where the probe does not contact the sample.
 This method is sometimes referred to as a “high
 intensity ultrasonic bath”. The cavitation within
 the water processes the micro tubes or vessels
 which are suspended during processing.
 Non-contact method is most effective for
 volumes less than 250 μl as foaming or sample
 loss are eliminated. In addition, this method
 ensures that sterile or pathogenic samples are
 not aerosolized preventing cross contamination

  

  Automatic Tuning : 

   All the of our Ultrasonic Homogenizers models employ a propriety feedback system,
   insuring that the generator is always working at it maximum efficiency regardless of the
   application. There is no need for constant adjustment by the operator.
   The percentage of ultrasonic power emitted is indicated by the output LED meter,
   enabling accurate reproducible results.

  

  Applications : 

Extract DNA / RNA

Extract Proteins

Immiscible Liquids

Dissolve Powders

Disperse Solids

De-gas Liquids

Deaglomerate Powders

Prepare Tissue

Prepare Emulsions

Dissolve Tablets

Emulsify Cosmetics

Emulsify Liposomes

Accelerate Enzymatic Reactions

Accelerate Chemical Reactions

Stimulate Bacterial Activity

 

             Models

  Catalogue number : 0-121-0002

 

   Descriptions

   The Model 150V/T Ultrasonic Homogenizer offers precision engineering with all the features necessary
   to create a total  system for 150 watts of ultrasonic disruption.

  

   Functions
   
PULSER POWER MODE – This allows the sample to be processed at full power while limiting

   temperature increase and is essential for heat sensitive samples.
   CONSTANT POWER MODE – Selectable for non-heat critical sample applications.
   
With 0-100% Amplitude (Power) control, the operator can optimize the titanium probe’s intensity to

   efficiently process the sample.

   TIMER – 1 to 15 minutes                  

 includes:
   - ultrasonic generator  
   - transducer assembly  
   - stainless steel transducer post  
   - laboratory clamp
   - 3 prong transducer clamp
   - pair of pin wrenches

  Specifications

  PROCESSING VOLUME

  250 µl - 300 ml

  TIMER

  1 - 15 MINUTES

  MAX. TIP DIAMETER

  1/2" (12.7mm)

  PULSER

  10 - 90%

  POWER OUTPUT

  0 - 150 WATTS

  DISPLAY TYPE

  LED

  OUTPUT FREQUENCY

  20 kHz  

  MICRO PROCESSING CONTROL

  N/A

  AUTOMATIC TUNING

  YES

  SOUND ABATING CHAMBER

  OPTIONAL

  

  Catalogue number : 0-122-0002

 

   Descriptions

   The Model 300V/T Ultrasonic Homogenizer offers precision engineering with all the features necessary

   to create a total  system for 300 watts of ultrasonic disruption.

   
   
Functions
   
PULSER POWER MODE – This allows the sample to be processed at full power while limiting

   temperature increase and is essential for heat sensitive samples.
   CONSTANT POWER MODE – Selectable for non-heat critical sample applications.

   With 0-100% Amplitude (Power) control, the operator can optimize the titanium probe’s intensity to

   efficiently process the sample.
   TIMER – 0 to 15 minutes          

 includes:
   -  ultrasonic generator  
   -  transducer assembly  
   - stainless steel transducer post  
   - laboratory clamp
   - 3 prong transducer clamp
   - pair of pin wrenches

  Specifications

  PROCESSING VOLUME

  250 µl - 2000 ml

  TIMER

  1 - 15 MINUTES

  MAX. TIP DIAMETER

  1" (25.4mm)/Cup Tip

  PULSER

  10 - 90%

  POWER OUTPUT

  0 - 300 WATTS

  DISPLAY TYPE

  LED

  OUTPUT FREQUENCY

  20 kHz  

  MICRO PROCESSING CONTROL

  N/A

  AUTOMATIC TUNING

  YES

  SOUND ABATING CHAMBER

  OPTIONAL

  

 Catalogue number : 0-127-0002

 

   Descriptions

   The Ultrasonic 3000 Ultrasonic Homogenizer delivers 300 watts of ultrasonic disruption and includes

   an integrated Sound Abating Chamber to reduce cavitational sound emitted during processing.
   The clear door permits viewing of the sample while processing. An access port for cables and tubing

   is also provided for use with Cup Tips and the Continuous Flow Chamber


   
Functions
   
PULSER POWER MODE – This allows the sample to be processed at full power while limiting

   temperature increase and is essential for heat sensitive samples.
   CONSTANT POWER MODE – Selectable for non-heat critical sample applications.

   With 0-100% Amplitude (Power) control, the operator can optimize the titanium probe’s intensity to

   efficiently process the sample.
   TIMER – 0 to 15 minutes         
 

 includes: 
  - sound abating chamber with     adjustable height sample table
  - transducer assembly      
  - pair of pin wrenches  

  Specifications

  PROCESSING VOLUME

  250 µl - 2000 ml

  TIMER

  1 - 15 MINUTES

  MAX. TIP DIAMETER

  1" (25.4mm)/Cup Tip

  PULSER

  10 - 90%

  POWER OUTPUT

  0 - 300 WATTS

  DISPLAY TYPE

  LED

  OUTPUT FREQUENCY

  20 kHz  

  MICRO PROCESSING CONTROL

  N/A

  AUTOMATIC TUNING

  YES

  SOUND ABATING CHAMBER

  INTEGRATED

  

 Catalogue number : 0-128-0002

 

  Descriptions

  3000MP Ultrasonic Homogenizer delivers 300 watts of ultrasonic disruption with precision control from

  a microprocessor and a graphical user interface displayed on a large bright 5.7" (145 mm) LCD display.
  The integrated Sound Abating Chamber reduces cavitational sound emitted during sample processing.

  The clear door permits viewing of the sample while processing. An access port for cables and tubing is

  also provided for use with Cup Tips and the Continuous Flow Chamber.

  With 0-100% Amplitude (Power) control, the operator can optimize the titanium probe’s intensity to

  efficiently process the sample.

     

  Functions
  MANUAL MODE   – the user defines the AMPLITUDE, can enable the PULSER feature, and monitor sample                              temperature during processing.
  PROGRAM MODE – the user can define, store, and recall up to 10 programs. Within each PROGRAM,

                             the AMPLITUDE, PULSER parameters, TEMPERATURE settings for COOL DOWN

                             and SHUT DOWN temperatures,  can be defined and stored.
  MACRO MODE     – the user to assemble up to 8 sequences using any of the 10 stored PROGRAMS with

                             an optional user defined time delay, from 1 secs to 99 hrs, between each sequence. 

 includes:  
   - Ultrasonic generator with    
     integrated sound abating
     chamber with adjustable
     height sample table
  - transducer assembly  
  - temperature probe
  - pair of pin wrenches

  Specifications

  PROCESSING VOLUME

  250 µl - 2000 ml

  TIMER

  1 SEC - 99 HOURS

  MAX. TIP DIAMETER

   1" (25.4mm)/Cup Tip

  PULSER

  1 SEC - 1 HOUR

  POWER OUTPUT

  0 - 300 WATTS

  DISPLAY TYPE

  5.7” (145mm)GLCD

  OUTPUT FREQUENCY

  20 kHz  

  MICRO PROCESSING CONTROL

  YES

  AUTOMATIC TUNING

  YES

  SOUND ABATING CHAMBER

  INTEGRATED

 

               Probe Tips & Accessories

Micro Tip Probes 300 μl to 25 ml Sample Volumes 

Micro Tips are narrow and long probes which generate high intensity cavitation for processing small  sample volumes.  The Stepped Micro Tip probe is coupled
directly to the Transducer.  The Tapered Micro Tip probes are threaded to the 1/2” (12.7 mm) Tapped Tip probe (0-120-0010) then coupled to the Transducer.

 

 

 Catalog No.

120-0005

120-0007 *

120-0008 *

 TIP DIA.(MM)

3.81

3.18

4.76

 VOLUME

300µl-15ml

500µl-10ml

750µl-25ml

 INTENSITY

VERY HIGH

VERY HIGH

HIGH

 AMPLITUDE(u)

210

220

190

 AMPLITUDE

50%

50%

50%

 LENGTH(CM)

25.58

158.50

104.14

 TYPE

STEPPED

TAPERED

TAPERED

 

Solid Tip Probes  5 ml to 2000 ml  Sample Volumes  

Solid Tip probes are manufactured from a titanium alloy and machined to a specific diameter and shape.
When coupled to the Transducer, the probe is driven to its resonant frequency, causing the probe to expand and contract longitudinally. When the probe is
immersed into a liquid  sample, the cavitation formed processes the sample. Solid Tip probes should be utilized when processing liquids containing organic
solvents or high surface tension samples. In addition, Solid Tip probes are machined from one solid titanium piece and less likely to cause sample cross
contaminations. Sample volume is determined by the tip diameter. Smaller diameter probes generate high intensity cavitation in a small focused area, ideal
for small sample volumes. Larger diameter probes generate low intensity cavitation in a broader area, ideal for larger sample volumes. The radiating face or
flat portion of the probe will pit or erode in time and should be replaced when worn. Probe performance degrades in proportion to the degree of erosion or
pitting which occurs at the tip surface, until a point is reached where the level of energy transmitted in the sample is significantly lessoned.

 

  

 Catalog No.

120-0009

120-0011

120-0013

120-0015

 TIP DIA.(MM)

9.52

12.7

19.05

25.4

 VOLUME

5ml-200ml

10ml-300ml

25ml-600ml

50ml-1000ml

 INTENSITY

HIGH

MED-HIGH

MEDIUM

LOW

 AMPLITUDE(u)

160

140

70

40

 AMPLITUDE

80%

100%

100%

100%

 LENGTH(CM)

10.79

13.65

13.02

12.70

 TYPE

 SOLID

SOLID

SOLID

SOLID

  

Tapped Tip Probes 10 ml to 2000 ml  Sample Volumes  

Tapped Tip probes are a 2 piece design comprised of the Body and Flat Tip, both manufactured from a titanium alloy and machined to a specific diameter and
shape. The body has a threaded end allowing the flat tip to be replaced. The flat tip or radiating face will pit  or erode in time and should be replaced when
worn. Probe performance degrades in proportion to the degree of erosion or pitting which occurs at the tip surface, until a point is reached where the level of
energy transmitted in the sample is significantly lessoned. Tapped Tip probes are NOT recommended when processing liquids containing organic solvents or
high surface tension samples.  Utilizing Tapped Tip probes with such samples are  likely to cause sample cross contaminations.

 

 

 Catalog No.

120-0010 *

120-0012 *

120-0014

 TIP DIA.(MM)

12.7

19.05

25.4

 VOLUME

10ml-300ml

25ml-600ml

50ml-1000ml

 INTENSITY

MED-HIGH

MEDIUM

LOW

 AMPLITUDE(u)

140

70

40

 AMPLITUDE

100%

100%

100%

 LENGTH(CM)

13.65

13.02

12.70

 TYPE

TAPPED

TAPPED

TAPPED

  

Extender Tip Probes Solid and Tapped 

Extender Tips are utilized to extend the length of a probe to reach into long necked vessels. They attach to the corresponding diameter Tapped Tip probes and
generally increase the overall length by 5” (127 mm).  Extender Tips offer the same sample volume and amplitude as their corresponding diameter Tapped Tip probes.
Extender Tips are available in Solid to Tapped  configurations. Tapped Extender Tips use the corresponding diameter replacement Flat Tips.

 

 

Titanium Solid Extender Tips

Titanium Tapped Extender Tips

 

 Catalog No.

120-0032

120-0034

120-0036

120-0033 *

120-0035 *

120-0037 *

 TIP DIA.(MM)

12.7

19.05

25.4

12.7

19.05

25.4

 LENGTH(CM)

10.79

13.65

 13.02

13.65

13.02

12.70

 TYPE

 SOLID

SOLID

SOLID

TAPPED

TAPPED

TAPPED

  

Flat Tips Replaceable tips for Tapped Tips Probes and Tapped Extender Tips 

Tapped Tip and Extender Tip probes use replaceable Flat Tips.
During normal use, the radiating face or Flat Tip of the probes erodes and becomes less effective over time.
Replace Flat Tips with corresponding Tapped Tip or Extender Tip diameter.

 

 

 Catalog No.

120-0016

120-0017

120-0018

 TIP DIA.(MM)

12.7

19.01

25.4

  

CUP Tips 

The Cup Tip offers non-contact homogenization of small volume samples in a high intensity ultrasonic bath. Samples are processed in sealed tubes or vials eliminating
aerosols and cross contamination. Multiple tubes or vials of sterile or pathogenic samples can be processed simultaneously. The titanium probe is mounted within an
acrylic body where the body is filled with water. Sample tubes or vials can be placed in the tray and suspended above the probe during processing. The cavitation in the
water processes the tubes or vials.  During sample processing, heated is generated, so inlets with barbed fittings can be connected to cold water or a Chiller to maintain
temperature and water level within the acrylic body.

 

 

                                     

 Catalog No.

 0-120-0019

0-120-0020

 TIP DIA.(MM)

 38.1

 

Chamber Vol

250 ml

8 tubes

Intensity

Medium

 

  

Continuous Flow Chamber 

The Continuous Flow Chamber permits in line or continuous processing of large volume batch samples. Batch sample volumes can be recirculated through the
chamber multiple times for desired sample results. Samples are passed through the chamber using one of the two bottom inlets. As the sample flows through
the cavitation chamber, the sample is processed. The processed sample exits the chamber through the two outlet ports. By recirculating the sample, adjusting flow rate
and cavitation intensity, the desired results can be achieved. During sample processing, heated is generated by the probe. Connecting the integrated cooling jacket to
a cold water source or Chiller, will maintain sample temperature during processing.

 

 Catalog No.

0-120-0026

Material

Stainless Steel

Oper Pressure

20 psi

Internal Vol

35 ml

 * REQUIRES THE 3/4” (19.1 mm) DIAMETER SOLID TITANIUM TIP CATALOG NO. 0-120-0013

  

SONABOX Sound Abating Chamber 

The SONABOX sound abating chamber reduces cavitational sound emitted during processing when used in conjunction with the Model 150VT and 300 VT Ultrasonic
Homogenizers. Harmonics are produced by the vessel walls and fluid surface, and can be discomforting to the user with extended operation. The SONABOX reduces
the harmonics by approximately 20-25 dBa. The clear acrylic door permits viewing of the sample while protecting the operator against accidental splashing. The height
of the sample table can be adjusted to vessel size and shape. Access ports for cables and tubing are also provided for use with the Cup Tip or Continuous Flow Chamber

 

 Catalog No.

0-125-0001

Sample Table

Adjustable Height

Chamber Doo

Clear Acrylic Door

Cable & Tubing Ports

2

Outside

26.7 cm W x 21.6 cm D x 59.7 cm H

Inside

24.8 cm W x 19.7 cm D x 45.1 cm H

  

 

  

 Small Volume Probe Map 

No.

VOLUME

DESCRIPTION

CATALOG NO

-

Transducer

2-122-0010

Based on tube vol

Cup Tip

2-122-0019

-

Microtube Tray

2-120-0021

300ul~15ml

5/32"(3.9mm)Stepped Micro Tip

2-120-0005

250ul~10ml

1/8"(3.2mm) Tapered Micro Tip

2-120-0007

750ul~25ml

3/16"(4.8mm)Tapered Micro Tip

2-120-0008

5ml~200ml

3/8"(9.5mm)Solid Tip

2-120-0009

10ml~300ml

1/2"(12.7mm)Tapered Tip

2-120-0010

 

1/2"(12.7mm)Flat Tip

2-120-0016

  

 Large Volume Probe Map 

No.

VOLUME

DESCRIPTION

CATALOG NO

-

Transducer

2-122-0010

10ml~300ml

1/2"(12.7mm)Solid Tip

2-122-0011

10ml~300ml

1/2"(12.7mm)Tapped Tip

0-120-0010

 -

1/2"(12.7mm)Flat Tip

2-120-0016

10ml~300ml

1/2"(12.7mm)Solid Extender Tip

0-120-0032

10ml~300ml

1/2"(12.7mm)Tapped Extender Tip

2-122-0033

25ml~600ml

3/4"(19.1mm)Solid Tip

0-120-0013

25ml~600ml

3/4"(19.1mm)Tapped Tip

2-122-0012

-

3/4"(19.1mm)Flat Tip

0-120-0017

25ml~600ml

3/4"(19.1mm)Solid Extender Tip

2-122-0016

25ml~600ml

3/4"(19.1mm)Tapped Extender Tip

0-122-0016

50ul~2000ml

1"(25.4mm) Solid Tip

2-122-0015

50ul~2000ml

1"(25.4mm) Tapped Tip

0-122-0014

 -

1"(25.4mm) Flat Tip

2-122-0018

50ul~2000ml

1"(25.4mm) Solid Extender Tip

0-120-0036

50ul~2000ml

1"(25.4mm) Tapped Extender Tip

0-122-0037

0.25L/min

Continuous Flow Chamber

0-120-0026

  

 

SONABOX Sound Abating Chamber

 250ml Cup Tip and 8 position Micro Tube
Tray shown attached to transducer assembly

 Continuous Flow Chamber with 3/4' Dia. Solid  
Titanium Tip and Tranducer assembly

 

 

bar002.png