BEADYPLEX combines simultaneous competitive immunoassays in the same single reaction.
The test uses unique reagents comprising mixtures of antibiotic-conjugated beads (assay competitors), broad- range antibiotic binders
(receptors and antibodies), and fluorescent secondary binders.
Each bead, individually encoded by its specific size and internal fluorescence, in combination with a primary binder, enables the detection
of well-defined groups of antibiotics.
In a first assay step the beads and primary binders are incubated with the sample extract. In the second assay step the labelled secondary
binders detect the remaining primary binders on the beads surface, thus generating the final assay signal.
The resulting “beads-binders” complexes are then characterized by the Flow Cytometer, which entails the classification of the beads by
discrimination of their sizes and internal fluorescence levels, and the measurement of external fluorescence intensities.
The presence of antibiotics from a particular family is identified by a signal decrease for the corresponding encoded bead, with respect
to a zero dose control sample.