|
Principle
Sulfasensor is a competitive test involving one specific generic monoclonal antibody with high affinity for most of sulfamide molecules.
Before the test, a rapid processing of the honey is needed to release the sulfamides that are chemically bound to the sugars of the honey
sample.
The test requires the use of two components :
-
The first component is a microwell containing predetermined amount of antibody linked to gold particles.
-
The second is a dipstick made up of a set of membranes with two capture lines. The “control” line is located above the “Test” line.
When the reagent from the microwell is re-suspended with a liquid processed honey sample, specific antibodies will bind the analytes
if present during the first incubation.
When the dipstick is dipped into the sample, the liquid starts running vertically on the dipstick and passes through capture zones.
When the sample is free of sulfamides, a colour development occurs at the “test” line, indicating the absence of sulfamides in the honey
sample. On the contrary, the presence of Sulfamides in the sample will not cause the coloured signal to appear at the “test” line.
|
