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  GENIUL

  Advanced PhotoActivation System!

 The latest innovation in Viable PCR for detecting viable bacteria in environmental samples

 

 - PhotoActivation Systemdead organism의 DNA/RNA를 neutralizes 시켜, PCR, Flow Cytometry,

   Fluorescence Microscopy를 이용하여 living cells의 DNA/RNA선택적으로 Detection할 수 있습니다.

 

 - PhotoActivation System allows the activation of a photosensitizer (PS) by low doses of harmless visible

   light of an appropriate wavelength (blue, red, and yellow) to excite the PS to its reactive triplet state,

   which will then generate reactive oxygen species that are toxic to cells.      

 

 

Ethidium Monoazide (EMA)

  - Ethidium Monoazide (EMA) mono doses microtubes for individual sample treatment.

  - In the detection and enumeration of microorganisms in clinical samples, environmental or food

    control by molecular biology techniques it crucial to differentiate live cells from dead cells.  

  - Single dose tube Ready to use Dry reagent, no use of DMSO.

  - EMA will turn the applicability of genetic techniques and applications in many fields.

     

 Features   

  This product has been formulated to meet the range of EMA concentration used

  in public scientific works, and contains EMA enough to treat 1000 μl of sample.

Sample ( μl)

EMA μM

 

1000

12,5

If this volume of sample is used, the final EMA concentration would be 12,5 μM. .

500

25

250

50

If the application needs more concentration of EMA, reduce the sample volume accordingly

125

100

 Applications

 - Clinical Microbiology and infectious disease evolution.

 - Pathogen Detection and health risk assessment   

   of environmental and food samples.

 - Susceptibility studies: biocides, toxic, antibiotics.

 - Microbial ecology.

 

Reaction buffers

 - For combining with photo reactive dyes ( i.e Propidium or Ethidium Monoazide)

 - One of the critical steps in all viable cell detection workflow by the means of PMA or EMA

   photoreaction, is sample and reagent incubation on darkness.  
 -
 During this treatment is very important to maximize reagent diffusion trough dead cells, spores,

   and cysts of microbial aggregates.

- On the other hand also is necessary to prevent viable cell damage, for this reason ionic strength,

  pH, nutrient level and aerobic/anaerobic balance needs to be under control.

 Models   These reaction buffers are compatible with vPCR, Flow Cytometry and Fluorescence Microscopy procedures.

 Reaction Buffer standardFor general purposes, neutral pH, standard ionic strength.

 Reaction Buffer plus

Contains anti clumping agents, cell membrane fluidising agents and a reduced level of nutrients.

Designed for stabilizing live cells but accelerating reagent diffusion trough dead cells.
Compatible with Enterobacteria and Mycobacteria.

 Reaction Buffer anaerobic

Contains anti clumping agents, minimum nutrient levels and provides a reducing environment compatible

with PMA or EMA based reagents. Designed for stabilizing anaerobic live cells but accelerating reagent diffusion

trough dead cells. Compatible with Bifidobacteriaceae

 

 Feature   

     Bifidobacterium longum cfu reduction during vPCR workflow

vPCR of dead M.smegmatis

 Bifidobacterium cells supspensions with  Reaction buffer anaerobic or standard,  were treated with a conventional vPCR  workflow.(50mM of PMA during 30 min,  PhAST Blue at 100% during 15 min).
 Reaction buffer anaerobic protects  anaerobic cells during
  vPCR work flow.

 Thermaly inactivated (95ºC, 30 min)

 Mycobacteria suspension.

 Untreated with PMA or treated with

 50 mM, 30 min. with Reaction Buffer

 standard or Reaction Buffer plus.

 PhAST Blue photoactivation 10 min, 100%.

 Reaction Buffer Plus completely reduces

 qPCR signal

 

Reaction Tube

 - The optimal performance of photochemistry reactions is highly related with the amount of light
   received by the sample. In this product the optical properties of plastics and the tube alignment
   trough the PhAST platform have been considered in order to obtain the best-adapted solution. 

 - High transparent 1,7 mL snap cap micro- centrifuge tubes.

 - Optimal performance for PhAST Blue platforms.

   

 

 Feature   

 Light transmittance at 460 nm (%).

Specifications

 GenIUL reaction tubes show the best light  transmittance at 460 nm (wavelength used for

 Propidium monoazide or Ethidium monoazide

 photo-activation).

 Compared with other suppliers these reaction

 tubes can obtain up to twofold better transmittance

 results. 

 Aseptic manufacture Nuclease free.
 Centrifuge up to 14000 rpm Compatible with the Dark box
 Packaged in a Polypropylene zip bag containing 100 tubes.
 Easy to handle on the bench top.
 Can be sterilized in the same bag by autoclave or microwave
 oven (3 min-750 W) 
 

 

PEMAX

 - Double dye technology in order to overcome the current limitations of viability PCR procedures.
   Nowadays, the paradigm is based in the cell membrane integrity of living cells as a unique
   differential factor from dead cells. However, as appointed in first reports, some treatment
   procedures can induce cells death without compromising membrane integrity.
-  This new reagent, combined with the appropriate reaction buffer, extends the concept of
   viability PCR to cells with intact cell membrane structure but also with capability to actively
   maintain bacterial homeostasis, as a result of active.

-  Combining both dyes with the correct reaction buffer, the DNA from dead cells (with damaged or not
   damaged membranes) will be neutralized; therefore only DNA from live cells will be detected by PCR.

  

 

 Feature   PEMAX™(Patent pending) - Reagent 2 vials of 0,5 mg   - 25 monodoses (TBC – Biomarker Kit)

  Double dye technology combines two photo-reactive molecules with different size and charge.

  The smaller molecule is capable of crossing cell membranes, but most of microorganism by the means of active efflux pumps, are able to revert

  this uptake. To allow the cells to maintain their homeostasis, are necessary larger incubation times and well defined reaction buffers.

  The second dye is needed to complete dead cell DNA neutralization when in the sample exist high amounts of dead cells. 

 

Bifidobacterium spp. qPCR detection Kit

 - Designed and validated for vPCR
 - Very stable reaction mix, it doesn't need ice during transport or during PCR setup. 
 - Includes DNA standard.
 - Includes qualitative internal PCR control.
 - Large PCR amplicon for ensuring the best vPCR resolution
 - Multiple Intercalating Dye Technology (MIDtech), for ensuring a better DNA binding profile in large
   amplicons.

    

 

 Feature   

     

                       Reagents (50 reactions) 

   Color code 

     Contents

5x Reagent mix (٭)

Green

Cap 275 μl

qPCR Assay (٭٭) 

Orange Cap

1 x 50 reactions

Standard DNA (Positive control of amplification and Standard curve)

Yellow Cap

1 x 50 reactions

RNase-Free water

White Cap

1500 μl

 (٭) Reagent mix: GenIUL proprietary dye blend and HOT FIREPol® qPCR Mix Plus
 (٭٭) Contains target-specific primers

 

The v-DNA reagent

- The v-DNA reagent combines in a unique step, cells lysis and purification.

- It has been designed in order to fix all viability dyes and PCR inhibitors present in the sample.

- v-DNA Reagent provides a fast and easy genomic DNA extraction procedure.

- No organic extractions, enzymatic digestions, or spin columns are needed, enabling very high DNA

  recovery from samples even in presence of PCR inhibitors.

- The DNA obtained is ready for use to perform PCR reactions or other molecular biology procedures.

- Validated for viability PCR workflow.

 

 Instructions   Bacterial DNA Purification from vPCR sample

Tube에 vPCR reagent

넣고 mix

샘플 Centrifuge

13000xg, 5 분

Tube에서 pettlet과 0.05ml 시약 남기고 상층액제거

V-DNA  튜브에

샘플이동

D-Bag에 잔여액

Drain

샘플 Vorterx

1000rpm, 5분

 

 DNA 보관 필요시  0.3mL 다른 튜브에

 이동하여, 보관

 

 

 

 

 

 샘플 가열

80℃, 10분

V-DNA buffer 첨가

0.6mL / sample.

   Vortex the sample

1000rpm, 2분

Centrifuge the sample

1000xg, 2 분

Sample is ready

for assay.

 

 Feature   

  The v-DNA reagent has been designed to obtain

  whole genomic DNA from bacteria and yeast

  suspensions from viability PCR sample treatments

  or from complex samples such as soil, stool,

  water (effluent form waste water treatment plants,

  cooling tower…), and enrichment broth cultures

  from food products

Product type

Sample amount

vPCR

500 μL

Soil

50 mg

Stool

25 mg

Waste water

Concentrate from 50 ml

Drinking water

Concentrate from 1 L

Food (EB)

50-100 μL

 

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