3AMINOSENSOR Milk

  Rapid 6 minutes strip test for Multiple detection

  3AminoSensor is a rapid assay in dipstick format detecting the contamination of milk samples by   aminoglycosides molecules. This kit is specific for Neomycin/Paromomycin, (Dihydro)-Streptomycin, and

  Gentamycin detectio

  The test requires the use of two components.

  The first component is a microwell containing predetermined amounts of specific antibodies with high

  affinity for Neomycin/Paromomycin, (Dihydro)-Streptomycin, and Gentamycin molecules, linked to gold particles.

  The second component is a dipstick made up of a membrane with four capture zones : a "control" line located

  above the test lines for Neomycin, Streptomycin and Gentamycin molecules.

  Principle

  For a valid test, the upper red control line has to be

  visible after the second incubation. The other three

  are the specific “test” lines placed below the control

  line. The line for Neomycin / Paromomycin is located

 below the Dihydro- and Streptomycin line while the

 line related to Gentamycin is located above it.

 When the reagent from the microwell is re-suspended

 with a milk sample, all monoclonal antibodies will

 bind the corresponding analytes if present during the

 first 3-minute incubation at 40°C.

 Afterwards, when the dipstick is dipped into the milk,

 the liquid starts running vertically on the dipstick and

 passes through capture zones. When the sample is

 free of antibiotics, a colour development occurs at the

 specific capture lines, indicating the absence of the

 targeted analytes in the milk sample. On the contrary,

 the presence of antibiotics in the sample will not

 cause any coloured signal to appear at the specific

 capture lines.

  How to Use    3AminoSensor is a competitive test involving generic monoclonal antibodies in one single operation

 Benefits  

 Good to Know  

  Aminoglycosides are one of the major classes of antibiotics used in veterinary medicine to treat animal infections.

  They are especially effective against gram negative bacteria and are frequently used alone and in association with other antibiotics under

  intramammarry administration to treat mastisis of dairy lactating cows.

  The detection of residual antibiotics such as Aminoglycosides in milk is of great concern to farmers, milk industry, regulatory agencies and

  consumers.

  The consumption of Aminoglycosides can indeed have several adverse effects (allergic reactions, nephrotoxicity and ototoxicity) and may

  induce the emergence of multi-resistant bacteria that does not respond to commonly used treatments for human illnesses.

  Although some microbial inhibition screening tests have a broad spectrum of antibiotics detection in milk, they are usually unable to

  detect Aminoglycosides at their European Maximum Residue Limit (MRL) concentrations.

  In contrast, some existing immunoassays such as ELISA are very sensitive but are time-consuming and specific for only one single

  Aminoglycoside compound due to the very limited structural similarities between the members of this antibiotic family.