Glass Bottom Dishes & Glass Bottom Plates

Glass Bottom Dishes & Glass Bottom Plates CELLVIS

Glass Bottom Dishes

Glass Bottom Plates

References

- Chambered Cover Glass (4,8) - 29 mm Glass Bottom Dishes

- 35 mm Glass Bottom Dishes - 4 Chamber Glass Bottom Dishes

- Glass top glass bottom dishes - 55 mm Glass Bottom Dishes

- 35 mm Micro Well Dishes more

- 6 Micro-well Glass Bottom Plates

- 6 well Glass Bottom Plates - 12 well Glass Bottom Plates

- 24 well Glass Bottom Plates - 96 well Glass Bottom Plates

- 384 well Glass Bottom Plates more

- Q & A - Publications

- Protocols

REFERENCES

Direct Visualization of De novo Lipogenesis in Single Living Cells
Two-component protein-engineered physical hydrogels for cell encapsulation
Cardioprotection of the aged rat heart by GSK-3 inhibitor is attenuated: age-related changes in mitochondrial permeability transition pore modulation
Two Distinct Ca2+ Signaling Pathways Modulate Sperm Flagellar Beating Patterns in Mice
The PINK1/Parkin pathway regulates mitochondrial dynamics and function in mammalian hippocampal and dopaminergic neurons
Lipooligosaccharide is required for the generation of infectious elementary bodies in Chlamydia trachomatis
Optical measurement of cycle-dependent cell growth
Virulence determinants in the obligate intracellular pathogen Chlamydia trachomatis revealed by forward genetic approaches
Low Temperature and Chemical Rescue Affect the Molecular Proximity of ΔF508-CFTR and ENaC
Visualizing Escherichia coli Sub-Cellular Structure Using Sparse Deconvolution Spatial Light Interference Tomography
Incorporation of a selective sigma-2 receptor ligand enhances uptake of liposomes by multiple cancer cells
Improved throughput traction microscopy reveals pivotal role for matrix stiffness in fibroblast contractility and TGF- responsiveness
Radioluminescence Microscopy: Measuring the Heterogeneous Uptake of Radiotracers in Single Living Cells
A WAVE2-Arp2/3 actin nucleator apparatus supports junctional tension at the epithelial zonula adherens.
Cell Encapsulating Biomaterial Regulates Mesenchymal Stromal/Stem Cell Differentiation and Macrophage Immunophenotype
Mechanisms of Xenogeneic Baboon Platelet Aggregation and Phagocytosis by Porcine Liver Sinusoidal Endothelial Cells
G-protein signaling leverages subunit-dependent membrane affinity to differentially control βγ translocation to intracellular membranes
Identification of Endoplasmic Reticulum Export Motifs for G Protein-Coupled Receptors
Metazoan-like signaling in a unicellular receptor tyrosine kinase
JunB Mediates Basal- and TGFb1-Induced Smooth Muscle Cell Contractility
Cross-talk between Tetraspanin CD9 and Transmembrane Adaptor Protein Non-T Cell Activation Linker (NTAL) in Mast Cell Activation and Chemotaxis*
Chronic Tempol treatment restores pharmacological preconditioning in the senescent rat heart
Characterization of mechanical and biochemical properties of developing embryonic tendon
Optically triggering spatiotemporally confined GPCR activity in a cell and programming neurite initiation and extension
The Effect of Z-Ligustilide on the Mobility of Human Glioblastoma T98G Cells
Mutations in Planar Cell Polarity Gene SCRIB Are Associated with Spina Bifida
A Bacteriophage Tailspike Domain Promotes Self- Cleavage of a Human Membrane-Bound Transcription Factor, the Myelin Regulatory Factor MYRF
SNP Detection in mRNA in Living Cells Using Allele Specific FRET Probes
Sustained Na+/H+ Exchanger Activation Promotes Gliotransmitter Release from Reactive Hippocampal Astrocytes following Oxygen-Glucose Deprivation
Identification of Novel CELSR1 Mutations in Spina Bifida
Simultaneous Measurement of Smoothened Entry Into and Exit From the Primary Cilium
Detection and Quantitation of Circulating Tumor Cell Dynamics by Bioluminescence Imaging in an Orthotopic Mammary Carcinoma Model
An Image-Based Genetic Assay Identifies Genes in T1D Susceptibility Loci Controlling Cellular Antiviral Immunity in Mouse
A Novel Alpha Kinase EhAK1 Phosphorylates Actin and Regulates Phagocytosis in Entamoeba histolytica
Spatiotemporal Stability of Neonatal Rat Cardiomyocyte Monolayers Spontaneous Activity Is Dependent on the Culture Substrate
A Plasmodium Phospholipase Is Involved in Disruption of the Liver Stage Parasitophorous Vacuole Membrane
Exosomes Are Unlikely Involved in Intercellular Nef Transfer
Receptor for Advanced Glycation End- Products Signaling Interferes with the Vascular Smooth Muscle Cell Contractile Phenotype and Function
In Vitro Coinfection and Replication of Classical Swine Fever Virus and PorcineCircovirus Type 2 in PK15 Cells
The ML1Nx2 Phosphatidylinositol 3,5- Bisphosphate Probe Shows Poor Selectivity in Cells
Quantitative Live Imaging of Human Embryonic Stem Cell Derived Neural Rosettes Reveals Structure- Function Dynamics Coupled to Cortical Development

Poly-lysine coating of glass bottom dishes and glass bottom plates

Materials :

Poly-lysine solution

Both poly-L-lysine and poly-D-lysine of MW between 30,000 - 150,000 can be used for coating.

Poly-D-lysine is recommended since it is not subjected to protease released from cells.

Prepare 1mg/ml stock solution by dissolving 100mg Poly-D-lysine hydrobromide(Sigma P-0899) in 100 ml water.

Filter sterilize the solution then store aliquots at –20℃.

Working solution is obtained by diluting the stock solution with sterile water to around 0.1mg/ml (0.05-0.5mg/ml).

Procedure :

1. Add working solution of poly-lysine into a glass bottom dish or glass bottom plate.

If cells will be cultured only on the glass surface, then only the glass surface needs to be covered with

poly-lysine solution.

2.Incubate for 1 hour at room temperature in a hood.

3.Remove poly-lysine solution. Poly-lysine solution can be re-used for a few times(depending on its concentration).

4.Rinse 3 times with PBS

5.Poly-lysine coated glass bottom dish or glass bottom plate can be kept at 4℃ for about a month.

Note that coating on glass surface is less stable than coating on plastic tissue culture dishes or plates.

Thus it is recommended to coat the glass bottom dish/plate right before cell culture.

Collagen coating of glass bottom dishes and glass bottom plates

Materials :

Rat tail collagen (type I) solution

Rat tail collagen I is available from many commercial vendors, such as BD Biosciences(cat# 354249),

Sigma (cat #C3867), Invitrogen (cat #A10483-01).

It is recommended to purchase collagen solutions, so that sterilization is not needed since filter sterilization

of collagen could be problematic.

Dilution the stock solution to 50 ug/ml with 0.02M acetic acid or 0.01M HCl.

Procedure :

1.Add solution (50 ug/ml) into a glass bottom dish or glass bottom plate at 5 ug/cm².

If cells will be cultured only on the glass surface, then only the glass surface needs to be covered with collagen

solution.

2.Incubate for 1 hour at room temperature in a hood.

3. Carefully aspirate the collagen solution.
(alternatively, the glass bottom dish/plate can be left in the hood to dry overnight, and the following

washing step is not needed)

4. Rinse 3 times with PBS or serum-free media.

5. Collagen coated glass bottom dish or glass bottom plate can be air dried and kept at 4℃ for about a month.

Note that coating on glass surface is less stable than coating on plastic tissue culture dishes or plates.

Thus it is recommended to coat the glass bottom dish/plate right before cell culture.

Fibronectin coating of glass bottom dishes and glass bottom plates

Materials :

Fibronectin solution

Human Fibronectin from BD Biosciences(cat# 354008) is diluted to 10-50ug/ml with Ca++ and Mg++ free PBS.

The concentration should be proportional to coating concentration, i.e, make 10ug/ml solution for 1ug/cm²

coating, 50ug/ml for 5ug/cm² coating, etc.

Make appropriate aliquots and store at -20℃.

Do not store in frost-free freezer, avoid multiple freeze thaws.

Procedure :

1.Add appropriate amount of fibronectin solution to culture surface.

If cells will be cultured only on the glass surface, then only the glass surface needs to be covered with the

fibronectin solution.

2. Incubate for 1 hour at room temperature.

3. Carefully aspirate the remaining solution.

4. Rinse with media or PBS.

5. Fibronectin coated glass bottom dish or glass bottom plate should be used immediately.

What are the thickness of glass #0, #1, #1.5, #1.5H, #2?

What is the spectral transmittance of the cover glass ?
Spectral Transmittance of the #1 cover glass used for our glass bottom dish/plate:
What kind of adhesive is used to adhere the dish and the cover glass? How can I detach the bottom glass?
We use a USP class VI adhesive to adhere the dish and the cover glass. This adhesive is nontoxic, and it is resistant to water and common fixing reagents such as methanol and ethanol. This makes our glass bottom dishes and glass bottom plates ideal for long term cell culture. The bad news is that the adherence between the dish and the cover glass is so strong, that it is very difficult to detach the cover glass from the dish.
What is the difference between the plastic surface and glass surface for growing cells?
Most plastic vessels used to culture attached cells are made from polystyrene. Since polystyrene is hydrophobic and most cultured cells can not attach to hydrophobic surface, plastic cell culture vessels are usually surface treated to introduce hydrophilic groups onto the surface to help the attachement of cultured cells. Glass surface on the other hand, does not need any pre-treatment to allow the attachment of the cultured cells. However the attachment usually is not as strong as that of surface treated polystyrene, our experience indicates that poly-lysine, collagen coating works much better on plastic than on glass. Consequently, poly-lysine or collagen coating on glass are less stable than poly-lysine or collagen coating on surface treated plastics.
What are the exact dimensions of your glass bottom dishes and glass bottom plates?
We have a detailed diagram of each of our glass bottom dishes and glass bottom plates, you can find them in the detailed description of each product. The dimension of the glass bottom plates conforms to ANSI/SBS standard.
What is the harmonized tarrif code of your products?
The harmonized tarrif code of our glass bottom dishes and plates is 3923.90.00